Electronic Supporting Information Direct in situ measurement of specific capacitance, monolayer tension, and bilayer tension in a droplet interface bilayer
نویسندگان
چکیده
Direct in situ measurement of specific capacitance, monolayer tension, and bilayer tension in a droplet interface bilayer
منابع مشابه
Direct in situ measurement of specific capacitance, monolayer tension, and bilayer tension in a droplet interface bilayer.
Thickness and tension are important physical parameters of model cell membranes. However, traditional methods to measure these quantities require multiple experiments using separate equipment. This work introduces a new multi-step procedure for directly accessing in situ multiple physical properties of droplet interface bilayers (DIB), including specific capacitance (related to thickness), lipi...
متن کاملAdsorption Kinetics Dictate Monolayer Self-Assembly for Both Lipid-In and Lipid-Out Approaches to Droplet Interface Bilayer Formation.
The droplet interface bilayer (DIB)--a method to assemble planar lipid bilayer membranes between lipid-coated aqueous droplets--has gained popularity among researchers in many fields. Well-packed lipid monolayer on aqueous droplet-oil interfaces is a prerequisite for successfully assembling DIBs. Such monolayers can be achieved by two different techniques: "lipid-in", in which phospholipids in ...
متن کاملVoltage control of droplet interface bilayer lipid membrane dimensions.
A novel approach to control the area of anchor-free droplet interface bilayer (DIB) lipid membranes is presented. Unsupported DIB lipid membranes are formed at the interface of phospholipid-coated aqueous droplets dispensed in dodecane oil. Using electrodes inserted into the droplets, an external voltage is applied which modulates the effective DIB area. Electrical (capacitance or current) and ...
متن کاملThe molecular mechanism of lipid monolayer collapse.
Lipid monolayers at an air-water interface can be compressed laterally and reach high surface density. Beyond a certain threshold, they become unstable and collapse. Lipid monolayer collapse plays an important role in the regulation of surface tension at the air-liquid interface in the lungs. Although the structures of lipid aggregates formed upon collapse can be characterized experimentally, t...
متن کاملWhat is the surface tension of a lipid bilayer membrane?
In a recent paper in this journal, Chiu et al. (1995) presented a molecular dynamics study of a planar lipid bilayer membrane in water with the explicit incorporation of a surface tension. Parallel to the membrane plane, a negative pressure of-100 atmospheres was applied, derived from the measured surface tension of a monolayer at an air/water interface. The same approach was put forward by Fel...
متن کامل